Clinical significance of mammalian target of rapamycin/70 ku ribosomal protein S6 kinasesignal pathway in gastric carcinoma
Chen Hongfang
Shan Xinfang
Han Hongbo
Guo Tianhua
Abstract：Objective To evaluate the mammalian target of rapamycin(mTOR)/70 ku ribosomal protein S6 kinase(P70S6K)in gastric carcinoma.Methods Reverse transcription-polymerase chain reaction and Western-blot were used to determine the expression of mTOR/P70S6K signaling pathway in cancerous and paracancerous tissues in 32 cases and 22 normal healthy tissues.Results The expression level of mTOR mRNA was markedly higher in the gastric carcinoma tissues(0.61 ± 0.11) than in the paracancerous tissues(0.43 ± 0.10) and normal stomach tissues(0.39 ± 0.07) ; the expression of P70S6K mRNA was also higher in the gastric carcinoma tissues (0.63 ± 0.13) than in the paracancerous tissues (0.45 ± 0.14) and normal stomach tissues (0.44 ±0.15),with a significant difference among groups (P ＜0.05).There was a positive correlation between mTOR mRNA and P70S6K mRNA expression in gastric carcinoma tissues(r =0.521,P =0.033).Compared to those in other two groups,the protein expression of mTOR,P70S6K in gastric carcinoma were significantly increased.The expression of mTOR/P70S6K signaling pathway in the gastric carcinom tissues was significantly correlated with the pathological grading and lymph node metastasis,but not with the diameter and gender.Conclusions The mTOR/P70S6K signaling pathway is specifically activated in gastric carcinom tissues.The mTOR/P70S6K signaling pathway might play an important role in the pathogenesis of gastric carcinoma.
Keywords：Gastric carcinomaMammalian target of rapamycin/70 ku ribosomal protein S6 kinaseReverse transcription-polymerase chain reaction
Publication Date：2014-11-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 1632-1634 )