The relationship between peripheral blood monocytes Toll-like receptor 2 gene expression and serum level of interleukin 4, interleukin 12 in asthma patients

Xue Min; Jiang Liyan; Gao Xiwen

doi:10.3760/cma.j.issn.1673-4777.2014.09.012

Abstract：Objective To investigate the expression of Toll-like receptor 2(TLR2) mRNA in monocytes of patients′blood during asthma control period , non-control period and healthy people;to investigate serum levels of interleukin-4 (IL-4) and IL-12,and to explore their correlation.Methods According to the standard of GINA 2006 asthma control level , 20 patients were selected for the asthma control period and 20 were selected for the non-control period during emergency and clinic treatment;20 healthy physical examiners were selected for the control group.Expression of TLR2 mRNA was detected by reverse transcription polymerase chain reaction ( RT-PCR) method;serum levels of IL-4 and IL-12 were detected by enzyme linked immunoassay ( ELISA ) .Results Ex-pressions of TLR2 mRNA of the asthma patients was higher than that in normal control group [(0.963 ±0.132) vs (0.632 ±0.172)] (P<0.01).There was no significant difference in the expression of TLR 2 mRNA during asth-ma control period and non-control period[(0.936 ±0.117) vs (0.991 ±0.147)] (P>0.05).Serum level of IL-4 in asthma patients was higher than the normal control group [(34.0 ±8.2)ng/L vs (9.8 ±2.7)ng/L] (t=13.87,P<0.01) with statistically significance .While IL-4 in asthma non-control period patients was higher than that in control period patients[(42.5 ±5.4)ng/L vs (29.4 ±4.2)ng/L] (t=8.53,P<0.01).Serum level of IL-12 of patients was lower than that of normal control group [(29.4 ±3.9)ng/L vs (55.8 ±6.1)ng/L] (t=20.54,P<0.01).While there was no significant statistical difference in the serum level of IL-12 between patients in asthma control period and non-control period [(28.7 ±4.5)ng/L vs (30.1 ±3.0)ng/L] (t=-1.16,P>0.05).Expression of TLR2 mRNA of asthma patients had a positive correlation with serum level of IL-4 ( r=0.532,P<0.01), but there was no obvious correlation to serum level of L-12 (r =-0.05,P >0.05). Conclusions Expression of TLR2 mRNA and IL-4 of the asthma patients is higher than that in the control group patients.Serum level of IL-12 of the patients is lower than the normal control .Expression of TLR2 mRNA of asth-ma patients has a positive correlation with serum level of IL-4, but has no obvious correlation to serum level of IL-12.