Low intensity ultrasound enhances chemotherapeutic efficacy on human pulmonary carcinoma cell line A549 in vitro
Zhou Yanqiu
Guan Jie
Tian Ying
Wang Hongwu
Weng Chunxiao
Abstract：Objective To investigate whether low intensity ultrasound enhances chemotherapeutic efficacy on human pulmonary carcinoma.Methods The human pulmonary carcinoma cell line A549 cell suspension was divided into four subgroups:routinely cultured A549 cells as control group; A549 cell suspension treated with cisplation group (final concentration was 5 mg/L) ; A549 cell suspension treated with low intensity ultrasound group (30 kHz,2.8 W/cm2,for five minutes) ; A549 cell suspension treated with combination of low intensity ultrasound (30 kHz,2.8 W/cm2,for five minutes) and cisplation group (final concentration was 5 mg/L).The A549 cell suspension treated with various treatments was incubated for 36 h.The A549 cell apoptosis ratio was detected using flow cytometry.Results The A549 apoptosis ratio was significantly higher in the combination of low intensity ultrasound and cisplation (7.84 ± 2.74) ％ than that in the subgroup of cisplation alone (5.06 ± 0.88) ％ (P =0.012) or low intensity ultrasound alone (2.71 ± 0.99) ％ (P =0.004).The apoptosis ratio of the combination of low intensity ultrosound and cisplation,and the ratio of the cisplation alone were all significantly higher than those in the control group (2.64 ±0.99) (P =0.010,0.004) ; there was no significance difference between low intensity ultrasound group and control group (P =0.055).Conclusion Low intensity ultrasound cam enhance the effects of chemotherapeutics on human pulmonary carcinoma cell line A549 in vitro.
Keywords：Low intensity ultrasoundHuman pulmonary carcinoma cell line A549ChemotherapeuticApoptosis ratio
Publication Date：2014-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 978-982 )