Effect of gene heme oxygenase-1 on human umbilical vein endothelial cell apoptosis induced by constant high blood glucose and intermittent high blood glucose
Zhu Xuxin
Chen Yongsong
Abstract：Objective To investigate the effect of heme oxygenase-1 (HO-1) on apoptosis of human umbilical vein endothelial cells (HUVECs) induced by constant high blood glucose and intermittent high blood glucose.Methods Untransfected HUVECs and transfected HUVECs were exposed for 7 days to 5.5 mmol/L lD-glucose (normal glucose),20.0 mmol/L D-glucose (constant high blood glucose) and 5.5 mmol/L alternating with 20.0 mmol/L D-glucose every 24 hours (intermittent high blood glucose),respectively.The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Cell apoptosis was detected by acridine orange (AO)/ethdium bromide (EB) staining.The mRNA expression of bax and bcl-2 was evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.Results Compared with untransfected HUVECs,the expression level of HO-1 mRNA was increased significantly in transfected HUVECs [(0.91 ± 0.29) vs (0.43 ± 0.14),P ＜ 0.05].Transfected HUVECs showed higher viability (108 ± 27,87 ± 9)than untransfected HUVECs(P ＜ 0.05).Transfected HUVECs had a lower apoptotic cells(35.0,48.0) than untransfected HUVECs(P ＜0.05).Transfected HUVECs had higher mRNA expression of bcl-2 compared with untransfected HUVECs.Conclusion The up-regulation of gene HO-1 protect HUVECs from apoptosis induced by constant high glucose and intermittent high glucose is associated with the up-regulation of bcl-2 expression.
Keywords：HyperglycemiaHeme oxygenase-1Endothelial CellsApoptosis
Publication Date：2014-06-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 826-830 )