Establishment of traumatic brain injury-induced stress ulcer model in rats with an electric cortical contusion impactor

Qiu Xiaowei; Cheng Shixiang; Tu Yue; Zhang Sai

doi:10.3760/cma.j.issn.1673-4777.2014.05.013

Abstract：Objective To establish of a traumatic brain injury-induced stress ulcer model in rats with an electric cortical contusion impactor (eCCI) for stress ulcer (SU).Methods A total of twenty Spraque-Dawley rats were selected and divided into sham group and brain injury group; each group had 10 cases.The parietal lobe brain cortexes in rats of brain injury group were given eCCI (4 m/s velocity for TBI,impact time was 200 ms,impact injury at 4 mm depth of penetration).Bone window of sham group was opened by dental drill (5 mm × 5 mm) after disinfection suture of the scalp.Regional blood flow of brain and gastric tissues in rats were determined by ultrasonic doppler blood flow meter after 48 hours of injury; histopathological changes of brain and gastric tissues were observed.Results Fourty eight hours after TBI,the mucosal blood flows in the brain surface in brain injury group were reduced compared with those in sham group (P ＜ 0.01) [(40.2 ± 1.9) ml/(min · 100 g) vs (107.6 ± 3.4) mL/(min · 100 g)].There was significant difference on regional blood flow between sham group and brain injury group(P＜0.01) [(32.9 ±2.0)ml/(min · 100 g) vs (42.1 ± 1.0)ml/(min · 100 g)].48 h after TBI,brain tissue of brain injury group was normal; structure was complete,the nucleus was stained blue and cytoplasm was pink.The number of neurons was decreased with structural disorder.The structure of gastric mucosal was disordered with mucosal surface cells and glandular stomach cell degeneration.Eosinophilic infiltrations of inflammatory cells in the sub mucosa of gastric were observed.Conclusions There is SU following TBI induced by electric cortical contusion impactor.The SU animal model induced by TBI in rats can be established successfully.