Effects of Rouganhuaxian grannules on matrix metalloproteinase 1 and matrix metalloproteinase 1 inhibitors in rats model of liver fibrasis induced by CCl4
Wang Zhenchang
Yang Shan
Huang Jingjing
Lv Jianlin
Abstract：Objective To investigate the effects of Rouganhuaxian grannules on matrix metalloproteinase 1 (MMP-1) and MMP-1 inhibitors (TIMP-1) in rats model of liver fibrasis induced by CCl4.Methods Liver fibrosis model was set up by CCl4,85 rats of health and sweeps were randomly divided into control group,pathologic model group,rouganhuaxian grannules group,Dahuang Zhechong pill group and autumn waters fairy alkali matched control group.The change of MMP-1 and TIMP-4 were check by immunol histochemical method and computer image-analysis technology.Results The severity of inflammation,liver fibrosis scorings and the level of collagen type Ⅳ in pathologic model group were (5.03 ± 2.66),(16.27 ± 6.21) and (194 ± 15),and all obviously higher than that in rats of control group (P ＜ 0.05).Compared with before treatment,the severity of inflammation,liver fibrosis scorings and the level of collagen type Ⅳ were obviously lower after treat for rouganhuaxian grannules group,Dahuang Zhechong pill group and autumn waters fairy alkali matched(P ＜ 0.05).The level of MMP1,T1MP-1 in pathologic model group were (1.30 ± 0.15) and (20.62 ± 4.56),and were all obviously higher than those in rats of control group [(0.94 ± 0.44),(10.52 ± 3.20)] (P ＜ 0.05).The level of MMP-1 in Rouganhuaxian grannules group was (1.58 ± 0.34),it was obviously higher than that in pathologic model group,Dahuang Zhechong pill group (1.39 ± 0.35) and autumn waters fairy alkali matched control group(1.41 ± 0.41) (P ＜ 0.05).But the level of MMP-1 in Rouganhuaxian grannules group was (13.6 ± 3.3),it was obviously lower than that in pathologic model group,Dahuang Zhechong pill group (18.3 ± 4.4) and autumn waters fairy alkali matched control group (17.3 ± 5) (P ＜ 0.05).The level of MMP-1 in Rouganhuaxian grannules group was (8.9 ± 2.0),it was obviously lower than that in pathologic model group,Dahuang Zhechong pill group (11.1 ± 1.9)and autumn waters fairy alkali matched control group(10.41 ±2.37) (P＜0.05).Conclusion Rouganhuaxian grannules can prevent and cure liver fibrasis through regulating the ratio of MMP-1/TIMP-1.
Keywords：Liver fibrasisRouganhuaxian granulesMatrix metalloproteinase 1Matrix metalloproteinase 1 inhibitors
Publication Date：2014-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：5( 512-516 )