Analysis of malignant and nonmalignant ascites after sample preparations in liver disease by differential expression of proteins

Zhang Yingjian; Wang Ping; Guo Hong; Zheng Yufeng; Su Xiuli

doi:10.3760/cma.j.issn.1673-4777.2014.04.018

Abstract：Objective To screen ascites biomarkers in posthepatitis cirrhosis and its relation to primary hepatic carcinoma patients with surface enhanced laser desorption /ionization time of flight mass spectrometry (SELDI-TOF-MS) technique.Methods Proteinic spectra was generated by SELDI-TOF-MS technique.The ascites from 46 posthepatitis cirrhosis(cirrhosis ascites group) and its related to 27 primary hepatic carcinoma patients (hepatic carcinoma ascites group)were analyzed for selection of the specific protein of liver cancer.The biomarker patterns were used.Results Before contration,the level of protein in cirrhosis ascites group was less than that in hepatic carcinoma ascites group [(8 ± 4) g/L vs (39 ± 4) g/L,P ＜ 0.05] ; after concentration,the level of protein in cirrhosis ascites group was close to the level in hepatic carcinoma ascites group[(54 ±5)g/L vs (58 ±5)g/L,P ＞ 0.05].Before centrifugation,the cell count of ascites in carcinoma ascites group was more than that in cirrhosis ascites group[(3 146 ± 1 362) × 106/L vs (287 ± 51) × 106/L] (P ＜ 0.05) ; the cell count of ascites in carcinoma ascites group and cirrhosis ascites group were 0.At the m/z was 4 475 and 11 467,the characterissic protein expression peak level was higher in carcinoma ascites group than that in cirrhosis ascites group(P ＜0.05),while at the m/z was 7 852,the characteristic protein expression peak level was less than that of cirrhosis ascites group (P＜0.05).The diagnosis way was established with the sensitivity of 81.5％ (22/27) and the specificity of 87.0％ (40/46).Conclusions Sample preparations for ascites proteome can meet the demands of surface enhanced laser desorption/ionization time of flight mass spectrometry.The characteristic protein expression peak has higher sensitivity and specificity.Differential expression protein method has important significance for further study of the ascites proteomic changes in the development and progression of liver cancer.