The evaluation of quality control on the new human IVF laboratory with mouse embryo testing
LI Hong
YUAN Cai-xia
QIN Qin
SONG Hai-xia
CAO Ying-li
SUN Xi-juan
Abstract：Objective To carry out the quality control of new human in vitro fertilization (IVF) laboratory,IVF of mouse were studied.Methods The gametes of mouse were got by surgery,treated by IVF and intracytoplasmic sperm injection(ICSI),the embryos were cultured for five days in vitro,fertility rate,grade 1-2 embryo rate and rate of cleavage to blastulas were investigated.Results 11 cycles of IVF were finished,271 eggs were gained,the fertility rate was 82.2％ and grade 1-2 embryo rate was 91.3％,the rate of 2-cell was growing to blastulas was 85.3％.Ten cycle of ICSI were finished,206 oocytes at metaphase Ⅱ (M Ⅱ)were gained,the fertility rate was 84.5％ and grade 1-2 embryo rate was 92.9％,the rate of 2-cell was growing to blastulas was 89.9％.Conclusion The quality control of culture condition in human IVF technology is tested by mouse embryo,the data accord with standards.
Keywords：Vitro fertilizationMouse embryo testQuality controlFertility rateGrade 1-2 embryo rate
Publication Date：2013-12-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：2( 47-48 )