Expression of E26transcription factor-1 in peripheral blood mononuclear cell of the patients with diabetic cardiomyopathy
WU Keng
YOU Qiong
LI Teng
LI Shang-hai
MO Hai-liang
YE Shao-qiang
HUANG Rui-na
Abstract：Objective To primarily discuss the role of E26 transcription factor (Ets) 1 in peripheral blood mononuclear cell of the patients with diabetic cardiomyopathy(DCM) and to provide the new measure to cure DCM.Methods DCM group (n =28) and type 2diabetic mellitus(T2DM) group (n =30),control group (n =30).The expression of Ets-1 mRNA on peripheral-blood mononuclear cells was examined by SYBR Green Ⅰ real-time quantitative reverse transcription ploymerse chain reaction.Results The levels of low density lipoprotein cholesterol (LDL-C) and triglyceride(TG) in DCM group were significantly higher than those in control group,the E/A in DCM group was significantly lower than that in control group [LDL-C:(3.3 ± 1.2) mmol/L vs (2.5 ± 0.8) mtol/L,TG:(3.5 ± 1.8) mmol/L vs (1.8 ± 0.6) mmol/L,E/A:(0.45 ± 0.14) vs (1.24 ± 0.16),P ＜ 0.05].The levels of fasting blood glucose(FBG) and glycosylated hemoglobin(Hb) A1c in DCM and T2DM groups were significantly higher than those in control group [DCM group (8.7 ± 3.6) mmol/L and (8.5 ± 2.6) ％,T2DM (9.0 ± 2.5) mmol/L and (9.0 ± 1.6) ％,control group (5.4 ± 1.2) mmol/L and (5.3 ± 1.2) ％,P ＜ 0.05].The E/A in DCM group was significantly lower than that in T2DM group(0.93 ±0.34) (P ＜0.05).The expression of Ets-1 mRNA on peripheral-blood mononuclear cells in DCM group were significantly higher than that in control group (0.58 ±0.17 vs 0.42 ± 0.15,P ＜ 0.05).The expression of matrix metalloproteinase(MMP) 9 in DCM group and T2DM group were significantly higher than those in control group [(3.7 ± 0.8) μg/L and (2.1 ± 0.6) μg/L vs (1.5 ± 0.5) μg/L,P ＜ 0.05],there were not statistically significant between DCM group and T2DM group (P ＜ 0.05).The expression of Ets-1 were correlated positively with MMP-9 in DCM (r =0.81,P =0.03),and there were not correlated positively with E/A,FBG,HbA1c,LDL-C and TG (r =-0.52,0.73,0.26,0.06,0.72,all P ＞ 0.05).Logistic regression analysis showed that Ets-1 was high risk factors of DCM [odd ratio (OR) =1.31,95％ confidonce interval (CI):2.14-4.48,P ＜ 0.05].Conclusion Ets-1 can up-regulate the expression of MMP-9 in the progress of DM,but only Ets-1 can be a predictor of DCM.
Keywords：Diabetic cardiomyopathyE26 transcription factor 1Matrix metalloproteinase 9
Publication Date：2013-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 1238-1240 )