Expression of mitofusin-2 in human vascular tissue and vascular smooth mucle cells
Abstract：Objective To observe expression of mitofusin-2 (Mfn2) in human vascular tissue and vascular smooth mucle cells.Methods Coronary heart disease patients underwent coronary artery bypass graft operation in Beijing Anzhen hospital from October 2006 to October 2007 were enrolled in this study and divided into hypertensive group(n =30)and normotensive group(n =30).During the operation,the redundat bridge vessels (internal mammary artery) were collected.Umbilical vein was collected from a neonatal infant by a normal pregnant woman.Vascular smooth muscle cells (VSMCs) from internal mammary artery and umbilical vein were cultured in vitro.Total RNA was extracted from explanted vessels and cultured VSMCs and Mfn2 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR).Using different concentraion of platelet-derived growth factor (PDGF-BB) to stimulate the cultured VSMCs and Mfn2 expression was determined by Semi Quantitative RT-PCR.Results Blood pressure and body mass index were significantly higher in hypertensive group compared to normotensive group(P ＜ 0.05).Mfn2 expression in VSMCs and vessel tissue were significantly lower in hypertensive group than those in normotensive group [(0.42 ± 0.10) vs (0.49 ± 0.12),P =0.014; (0.29 ± 0.07) vs (0.33 ±0.08),P =0.041].Add PDGF-BB to normal human VSMCs with certain concentraion,expression of Mfn2 decreased and the proliferation of VSMCs increased.Conclusion In vascular tissue and cultured VSMCs,there are expression differnces of Mfn2 between hypertensive patients and normotensive patients.
Keywords：Essential hypertensionMitofusin-2Internal mammary arteryVascular smooth muscle cells
Publication Date：2013-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 772-774 )