Establishment and pathological observation on the pulmonary hypertention model in rats with monocrotaline injection and left pneumonectomy
LIU Yang
LI Gang
LIU Ai-jun
ZHU Yao-bin
LI Xiao-feng
LI Zhi-qiang
LIU Ying-long
Abstract：Objective To establish the model of pulmonary hypertention in rats with mon0crotaline(MCT) injection and left pneumonectomy to observe the pathologic changes.Methods Twenty-two sprague dawley (SD) male rats of specific pathogen free (SPF) grade were randomly divided into two groups:control group (sham operated and sodium chloride injection; group B:12 rats) and left pneumonectomy and MCT injection group(group A:10 rats).The mean pulmonary arterial pressure (mPAP),fight ventricular hypertrophy index (RVHI),the tunica media thickness percentage of small pulmonary arteries(WT％) and index of blood vessel were examined in the rats after 4 weeks.The differences between the two groups with the fight lung and cardiac tissues were compared.Results The mPAP in group A increased significantly compared with that in group B [(42.4 ± 2.8) mm Hg vs (19.0 ± 2.6) mm Hg,P ＜ 0.05].Pathological changes such as fight ventricular hypertrophy were found in the rats 4 weeks after left pneumonectomy and MCT injection,significant pathological changes occurred including fight ventricular fibrosis and thickening of the tunica media of the pulmonary artery were also found.RVHI,WT％ and index of blood vessel increased significantly [(0.61 ± 0.10) vs (0.27 ± 0.05),(4.52 ± 0.19) vs 0,(26.38 ±4.27) vs (1.82 ± 0.03),P ＜ 0.05].Conclusion Left pneumonectomy and MCT injection can induce the model of pulmonary hypertention,and there were significant pathologic differences of lung tissues between the two groups.
Keywords：HypertensionpulmonaryMonocrotalinePneumonectomyRat
Publication Date：2013-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 318-320 )