Inhibition of nuclear factor-κB activation by naringin in diabetic cardiomyopathy rats
WU Keng
TU Yan-ming
YOU Qiong
HUANG Rui-na
LIANG Jian-guang
Abstract：Objective To investigate the effects of naringin on nuclear factor (NF)-κB inflammatory signaling pathway in diabetic cardiomyopathy rats,and to elucidate its cardioprotect mechanism in diabetic cardiomyopathy.Methods Diabetes cardiomyopathy rat model was established by high fat and sugar feed and one-time intraperitoneal injection of low dose streptozotocin.They were divided into model control(MC) group and naringin treatment group; the other set of normal control (NC) group.Expression of NF-κB in myocardium by immunohistochemistry and tumor recrosis factor α (TNF-α),interleukin (IL)-6,IL-1β in serum by radio immunoassay were detected.Results Compared with the control group,positive vascular density of NF-κB in myocardium and TNF-α,IL-6,IL-1β in serum were significantly increased in diabetic cardiomyopathy group,and the level of NF-κB in myocardium and the level of TNF-α,IL-6,IL-1β in serum was decreased in naringin treatment group[(0.162 ± 0.013) μg/L vs (0.222 ± 0.018) μg/L,(1.35± 1.02)μg/L vs (1.98 ± 1.37)μg/L,(15.8 ± 14.6)μg/L vs (35.8 ±21.1) μg/L,(0.14 ± 0.15) μg/L vs (0.27 ± 0.23) μg/L,all P ＜ 0.05].Electron microscopy results showed that myocardial ultrastructure was significantly injured.Naringin pretreatment alleviated myocardial ultrastructure changes with regular myofibrillar organization,intact membrane and cristae.Conclusion Naringin can ameliorate myocardial ultrastructure in diabetic cardiomyopathy ;the mechanism is possible related to NF-κB inflammatory signaling pathway in diabetic cardiomyopathy rats.
Keywords：DiabetesCardiomyopathyNaringinNuclear factor-κBInflammation
Publication Date：2013-03-30
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 295-297 )