Preventive and protective effect of AnNao pill on oxygen and glucose deprivation injury of rat cortical neurons
HAN Miao-miao
TANG Xue-jun
PEI Liang-yan
MEI Yuan-wu
Abstract：Objective To investigate the protective effects of AnNao pill on oxygen and glucose deprivation (OGD) in cultured rat cortical neurons.Methods Cultured primary cortical neurons were pretreated or not pretreated with AnNao pill medicated serum and were exposed to the oxygen and glucose deprivation (OGD) model.The results on cell viability rates,the apoptosis rates of neurons,the expression of Bcl-2,caspase-12,glucoseregulated protein (GRP) and cytochrome C (CytC) were compared between OGD and AnNao pill groups.Results Compared to the normal group,the rates of apoptosis [(22.0 ± 2.2) ％ vs (3.2 ± 0.6) ％] and the expression of caspase-12,GRP78 and CytC in the OGD group increased (all P ＜ 0.05) ; The rates of cell viability [(62.4 ±4.6) ％ vs (100.0 ± 3.1) ％] and the expression of bcl-2 decreased (all P ＜ 0.05).Compared to the OGD group,the viability rates in low dose,middle dose and high dose AnNao pill groups increased[(74.9 ±2.7)％,(83.0 ±2.2) ％,(75.2 ± 2.8) ％ vs (62.4 ± 4.6) ％] (all P ＜ 0.05).The rate of viability in middle dose AnNao pill group was higher than those in low dose and high dose AnNao pill group (all P ＜ 0.05).the rates of apoptosis [(10.4 ± 0.7) ％ vs (22.0 ± 2.2) ％],the expression of caspase-12 and CytC in the middle dose AnNao pill group were lower than those in the OGD group(all P ＜ 0.05) ; the expression of Bcl-2 were higher(all P ＜ 0.05)than that in the OGD group.Conclusion AnNao pill has a protective effect on rat cortical neurons against OGD and its mechanism may be that the AnNao pill can regulate associated protein of apoptosis and inhibit the apoptosis.
Keywords：Oxygen and glucose deprivationCell apoptosisGlucose-regulated protein 78Cytochrome CAnNao pills
Publication Date：2013-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 176-178 )