Low intensity ultrasound enhances cisplation chemotherapeutic efficacy on human hepatoma cell SMMC7721 in vitro and in vivo
LIU Zhao-yang
WANG Zhen-chao
LI Tao
SONG Li-jing
WENG Chun-xiao
Abstract：Objective To investigate the effects of low intensity ultrasound and synergism of combined with cisplation on human hepatoma cells.Methods The human hepatoma cells line SMMC7721 was treated with low intensity ultrasound,30 kHz,1.4 W/cm2 or 2.8 W/cm2.The killing effect of low intensity ultrasound on the cells was detected by the trypan blue exclusion and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyhetrazolium bromide (MTT) survival assay.The inhibitive tumor experiment study on mice was performed for cisplation combined with low intensity ultrasound 30 kHz,2.8 W/cm2.Results The killing effect of low intensity ultrasound (30 kHz,1.4 W/cm2 and 2.8 W/cm2) on human hepatoma cells was observed after 2 minutes or 5 minutes treatment.Most of the cells were killed by low intensity ultrasound combined with cisplation treatment.Compared with the application of cisplation alone,the cisplation antitumor activity was increased from an inhibitive rate of tumor 56.7％ to 84.9％ in MTT survival assay when cisplation was combined with a low intensity ultrasound at 30 kHz,2.8 W/cm2,5 minutes in vitro.Conclusions The low intensity ultrasound wave can kill human hepatoma cells and increase antitumor activity efficiency when it is combined with cisplation.
Keywords：Low frequency ultrasoundHuman hepatoma cellCisplatinSynergism
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：4( 1538-1541 )