Effects of extracts of Xuelian on nitric oxide synthase isoforms in cortex region of cerebral ischemia-reperfusioninjury in mice
DANG Hui
LI Jian
BU Juan
AIshanjiang
ZHU Yi
Mayinuer
SHA Jing
LI Hong-yan
JING Yan
Abstract：Objective To investigate the effect of extracts of Xuelian on the expression of the three different isoforms in cortex region of cerebral ischemia-reperfusion injury in mice.Methods A total of 32 institute of cancer research (ICR)mice were randomly divided into sham group,saline group,Xuelian group and edaravone group.Saline,Xuelian injection and edaravone was administered in each group by intraperitoneal injection for 7 days before transient middle cerebral artery occlusion (tMCAO)was performed.Immunohistochemistry staining was done after 24hours of reperfusion,and the mean numbers of positive cells of nNOS,iNOS and eNOS in the cortex regions were calculated.Results The numbers of Neuronal Nitric Oxide Synthase(nNOS),Inducible Nitric Oxide Synthase(iNOS)and Endothelial Nitric Oxide Synthase(eNOS)positive cells at 24 hours of reperfusion were (14.0 ± 4.8)/ HP,(15.0 ± 5.5)/HP and (18.2 ± 5.5)/HP respectively,which were significantly up-regulated compared to the sham group [(2.1 ± 0.8)/HP,(1.3 ± 0.6)/HP and (3.3 ± 1.9)个/HP] (all P =0.000).At 24 hours after reperfusion,the numbers of nNOS and iNOS positive cell in Xuelian group were(7.5± 3.8)/HP and (7.1 ±3.7)/HP,which was significantly less compared to saline group (all P =0.000).However,there was no significant difference in expression of eNOS positive cell (22.3 ±2.3)/HP compared to saline group (P =0.072).Conclusions It is suggested that extracts of Xuelian can exert neuroprotective effects through down-regulation of expression of nNOS and iNOS in cortex after cerebral ischemia-reperfusion injury.
Keywords：Cerebral ischemia-reperfusionNeuronal nitric oxide synthaseInducible nitric oxide synthaseEndothelial nitric oxide synthase
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)