Effect of phenytoin on intracellular concentration of carmustine and teniposide in chemotherapy drug-resistant glioma cells
L(u) Hua-rong
WANG Jun
WU Xing
ZHOU Bin-yin
DAI Xue-yuan
PEI Yong-en
Abstract：Objective To investigate the concentration of carmustine and teniposidc in glioblastoma cell line 8-MG-BA and H4 after incubated in different concentrations of Phenytoin (PHT)in vitro.Methods After treatment with phenytoin on different concentrations,intracellular concentration of carmustine and teniposide in H4 and 8-MG-BA cell line was determined by high efficiency liquid chromatography method in 60 and 120 minutes.Verapamil was used in control group.We used external standard calibration curve method to make linear regression calculations and the instrument precision and recovery rate of the methods were determined.Results The intracellular concentration of of bis-chloroethyltrosourea (BCNU)and teniposide (VM-26)in H4 cells was significantly higher than that in 8-MG-BA cells in control group (P ＜0.01).After being treated with PHT 5 mg/L,10 mg/L and VRP5 mg/l,the intracellular concentration of BCNU and VM-26 in 8-MG-BA cells was significantly higher than that in control group (P ＜ 0.05).The intracellular concentration of of BCNU and VM-26 in cells treated with PHT 5 mg/L.was significantly lower than that in PHT 10 mg/L treatde group (P ＜ 0.05).When the drug mass concentration was 0.05 to 5 mg/L,there was a good linear relationship between drug concentration and peak area.The recovery of VM-26 was (96 ±5)％,(100±4)％,(99 ±2)％ respectively.The recovery of VM-26 was (100 ± 5)％,(99 ±4)％,(99 ±4)％ respectively.The intra and inter-day RSD were less than 5％ (n =5).Conclusion PHT can increase the intracellrlar concentration of BCNU and VM-26 in 8-MG-BA cells.
Keywords：GlioblastomaPhenytoinDrug concentrationHigh performance liquid chromatography
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)