The effects and mechanisms of ambroxol pretreatment on rats' gastric aspiration lung injury
XU Jun-ma
ZHANG Yan-min
JIA Xiao-min
ZHAO Jie
Abstract：Objective To investigate the effects and mechanisms of ambroxol pretreatment on rats' gastric aspiration lung injury.Metbods Thirty Sprague-Dawley rats were randomly divided into three equal groups:eontrolgroup,injury group and ambroxol group.In the control group,physiologic saline was instilled into the trachea of the rats;in the injury group,gastric content was instilled into the trachea of the rats;in the ambroxol group,ambroxol was injected before the injury was carried out.Wet-to-dry weight ratio(W/D) of lung and the activity of myeloperoxidase(MPO) were measured;tumor necrosis factor-or(TNF-α) and interlcukin-lβ(IL-Iβ) of scrum and lung homogenate were examined by enzyme linked immunosorbent assay(ELISA).Toll like receptor 4(TLR4) was examined by Western blot.Lung pathological change was observed by microscope.Results Compared with control group,W/D of lung,the activity of MPO,TNF-α and IL-1β3 of serum/lung homogenate,TLR4 expression in Injury group increased[(5.01±0.22)vs(2.42±0.12),(3.50±0.24) U/g vs(1.10±0.18) U/g,serum:(0.950±0.260) μg/L vs(0.075±0.010) μg/L,(0.492±0.046) μg/L vs(0.009±0.001)μg/L;lung:(0.860±0.160)μg/L vs(0.080±0.011)μg/L,(0.583±0.054)μg/L vs(0.007±0.002)μg/L,p ＜0.05].Compared with injury group,W/D of lung(3.92±0.12),the activity of MPO [(2.76±0.52) U/g ],TNF-α and IL-1 β of serum/lung homogenate and TLR4 expression(1.24±0.15) in ambroxol group decreased(P ＜ 0.05).The microscopic structure of the control group was normal and the pathological changes of ambroxol group were milder than those of injury group.Conclusions Ambroxol pretreatment shows protective effects on gastric aspiration lung injury.The mechanism may relate to down-regulation of TLR4 and inhibit the expression of pro-inflammatory cytokins.
Keywords：Lung injuryAmbroxolToll like receptor 4
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)