Effect of tumor necrosis factor-α on expression of skeletal muscle E3 ubiquitin ligases atrogin-1 in rats with chronic obstructive pulmonary disease model
WANG Hui
LUO Yong
HAN Feng-feng
ZHANG Yue
YANG Tian-yun
XU Wei-guo
Abstract：Objective To study the expression of skeletal muscle E3 ubiquitin ligases atrogin-1 and the effect of tumor necrosis factor-α(TNF-α) on muscular atrophy in rats with chronic obstructive pulmonary disease(COPD) and discuss the mechanism of muscular atrophy of COPD rats.Methods Thirty SD rats were randomly divided into a control group(group A),a COPD group(group B) and a COPD + TNF-α group(group C),with 10rats in each group.After COPD models were eslablished by passive cigarette smoking in group B and C,the extensor digitorium longus muscles(EDL) were dissected and isolated in vitro muscle incubation system with adequate oxygen supply.The EDL were protein levels were delcrmined by real-lime quantitative PCR and Western blol respectively.Results The expressions of the Atrogin-1 mRNA of the group B and the group C were(1.475±0.272)times and(2.048±0.441) times more than that of control group(all P ＜ 0.01),meanwhile the level of expressions of the Atrogin-1 mRNA of group C was significantly higher than that of group B(P ＜ 0.05).The level of Atrogin-1 protein in group B and C were distinctly higher than that in group A(0.803±0.090) and(0.965±0.160) vs(0.626±0.142),all P ＜ 0.01,and the difference between group B and C was significant(P ＜ 0.05).Conclusion Atrogin-1 level in skeletal muscle of COPD model increases,which can be enhanced by TNF-α.The level of Atrogin-1 prolein in group B and C were dislinctly higher than that in group A(0.803±0.090 and 0.965±0.160 vs 0.626±0.142,all P ＜0.0 1),and the difference between group B and C was significant(P ＜0.05).
Keywords：Pulmonary diseasechronic obstructiveTumor necrosis faclor-αSkeletal muscleUbiquitin-dependent proteolytic pathway
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)