The effect of cathepsin S inhibitor on the apoptosis of rabbit vascular smooth muscle cells
ZHANG Yao-zhong
HUANG Fang-jiong
QIN Yan-wen
CUI Wei
CAI Lun
WANG Lu-ya
XIN Yi
Abstract：Objective To observe the effects and mechanisms of Cathepsin S(CTSS) inhibitor on the apoptosis of vascular smooth muscle cells(VSMC).Methods Vascular smooth muscle cells of rabbit were primary cultured from aortic tissue and subcultured.VSMCs were divided into control group,one drug group(angiotensin Ⅱ)and combined drugs group(CTTS inhibitor + angiotensin Ⅱ);we used flow cytometry and TUNEL to detect the change of apoptosis of VSMC.Western-blotting was applied to detect the expression of apoptosis related protein Bcl2,Bax,Caspase-3.Results ①Both flow cytometry and TUNEL showed that at the time points(10h,12h),compared with one drug group,the apoptosis of VSMC in combined drugs group alleviated remarkably,the difference was statistically significant[ 10b:(23607±176) vs(36058±269),12h:(29813±205) vs(39146±263),both P＜0.05 ];②Western-blotting:compared with one drug group,combined drugs group could down-regulate the ratio of the expression of the apoptosis related protein Bax/Bcl-2 obviously(P ＜ 0.05);the expression of apoptosis related protein Caspase-3 reduced obviously(P＜0.05).Conclusion Cathepsin S inhibitor may alleviate the apoptosis of VSMC by down-regulating the expression of apoptosis related protein BAX and Caspase-3.
Keywords：Myocytessmooth muscleCathepsin SCell apoptosis
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)