Effect of remote ischemic preconditioning on intestinal ischenia-reperfusion injury
XING Li
PAN Xu-dong
L(U) Ying-qian
ZHAO Li
LI Hu-chen
Abstract：Objective To investigate the effect of remote ischemic preconditioning (RICP) on intestinal is-chemia-reperfusion (I/R) injury in a rat model.Methods The model of remote ischemic preconditioning in rats was made by 3 × 5-min cycles of right upper arm/leg ischemia and 5-min reperfusion.Then intestinal I/R was established by clamping both the celiac trunk and the superior mesenteric artery for 30 min,foflowed by reperfusion for 2h.The animals were assigned to different groups:I/R group,RICP group and Sham group.The morphological changes of intestine,PARP-1 activation,apoptosis were detected by means of HE,immunohistochemical staining for poly (ADP-ribose) and TUNEL.Regional inflammatory reaction and lipid peroxidation were indicated by myeloperoxidase (MPO) activity and malonaldehyde (MDA).Results In I/R group,the extent of intestinal injure and PARP-1 activation,Compared with Sham group,TUNEL positive-staining,MPO activity and MDA were elevated significantly in I/R group [ (4.65 ± 0.82) vs (0.56 ± 0.48 ),( 23.8 ± 2.8 ) ％ vs ( 5.1 ± 0.8 ) ％ ; ( 330 ± 15 ) U/g vs (24 ±5)U/g;(248 ±15)μnol/(L · mg) vs (36 ±7)μmol/(L · mg),P＜0.05) ].In RICP group,these variables [ (2.92 ±0.74),( 16.8 ±3.1)％,(218 ± 17)U/g,(121 ± 13) μmol/(L · mg) ] were statistically lower than those in I/R group( P ＜ 0.05 ),but higher than those of Sham group ( P ＜ 0.05 ).Conclusion These findings demonstrat that RICP has the potential protection from intestinal I/R,which could be induced by inhibiting regional inflammatory reaction,lipid peroxidation and PARP-1 activation.
Keywords：Ischemia-reperfusionIntestinal injureRemote ischemic preconditioningPoly ( ADP-ribose) polymerase-1
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)