The feasibility to prepare cytokine-induced killer cells using the lymphocytes removed from O type blood in the blood bank
FAN En-yong
SUN Hai-ying
GUO Ming-hua
JIAO Yu-dong
Abstract：Objective To investigate the feasibility of preparing cytokine-induced killer cells (CIK) using the lymphocytes removed from O type blood in the blood bank.Methods The whole blood was collected from 30 blood donors with O type blood.The white blood cells were enriched and were seperated by density-grediant centrifugation.The lymphocytes were differentiated,induced and cultured to prepare CIKs.Results The CIKs produced from the 30 cases of blood donors with type O blood was ( 1.30 ±0.12) × 1010/L with CD3 +/CD56 + population up to 40％ ～ 60％.The recovery rate was up to over 90％ after 1 month of cryopreservation.Conclusion Preparing the CIK with the lymphocytes removed from the type O blood donation is an ecnomical way to cut the cost of CIK preparation.
Keywords：Voluntary blood donationWhite blood cellsLymphocytesCytokine-induced killer cells
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)