The effect of oolong tea ingredients on liver cancer

JIA Hai-mei; ZHENG Neng-xiong; WANG Jing-quan; YAO Xu; CHEN Yang-wei; LIN Li-fang; WANG Li-hong

doi:10.3760/cma.j.issn.1673-4777.2012.03.022

Abstract：Objective To study the effect of oolong tea and tea ingredients on liver cancer.Methods The human hepatoma cell line G2 (HepG2) was incubated in RPMI1640.HepG2 was exposed to oolong tea and tea polyphenol,tea pigment,theanine and tea polysaccbaride at the different concentrations of 25 mg/L,50 mg/L,100 mg/L and 200 mg/L for 24 hours,respectively.The growth inhibition of HepG2 cells was measured by WST-1 assay.Results The concentrations of tea in the 100 mg/L and 200mg/L groups were significantly lower than those in control group(P ＜0.05).The concentrations of tea polyphenol,tea pigment in 25 mg/L,50 mg/L,100 mg/L and 200 mg/L groups were significantly lower than control group(0.2017 ± 0.0018 ) (P ＜ 0.05 ).The concentrations of theanine and tea polysaccharide in the 50 mg/L(0.1595 ±0.0019,0.1728 ± 0.0040),100 mg/L(0.1453 ± 0.0017,0.1555 ± 0.0045)and 200 mg/L groups(0.1305 ± 0.0024,0.1405 ± 0.0013 )were significantly lower than those in control group( P ＜ 0.05 ).The concentrations of tea polyphenol ( 0.1658 ± 0.0025 ) and tea pigment ( 0.1913 ±0.0013 ) groups were significantly lower than those in tea group in the 25 mg/L (0.2005 ± 0.0013 ) ( P ＜ 0.05 ).The concentrations of tea polyphenol(0.1545 ± 0.0041 ),tea pigment (0.1655 ± 0.0039 ),theanine (0.1595 ± 0.0019 ) and tea polysaccbaride(0.1728 ± 0.0040) were significantly lower than those in tea group(0.1970 ± 0.0078 )in the 50 mg/L( P ＜0.05 ).The concentrations of tea polyphenol (0.137 ± 0.0016)were significantly lower than those of tea group in the 100 mg/L(0.160 ± 0.0037)( P ＜ 0.05 ).Conclusions The results indicate that oolong tea and tea ingredients can inhibit the proliferation on HepG2 cells.It is suggested that oolong tea and tea ingredients can be used to treat human liver carcinoma.