The influence of metformin on adanced glycation end products induced by the vascular smooth muscle cell proliferation
ZHANG Yan-hua
Abstract：Objective To explore the influence of metformin on adanced glycation end products (AGE) induced by the vascular smooth muscle cells (VSMC) proliferation.Methods The VSMC were cultured by passaged cell cultured method; 100,200,400 mg/L of advanced glycation end products were acting on VSMC respectively,and located 200 mg/L of BSA group and blank control group; 10 -5mol/L,10 -4mol/L,10 -3mol/L of metformin +200 mg/L of AGE were acting on VSMC respectively,and observed the proliferation changes at 12,24,48 and 72 h after dosing the drugs; the absorbance value was determined by MTT method.Results The different concentrations of AGE group can promote proliferation of VSMC at different times; and 100 mg/L of AGE group had proliferation effect to VSMC,at 12 h,200 mg/L of AGE group had the most obvious,and it had the strongest effect at 24 h;there was no statistically significant difference between the 400 mg/L group and the 200 mg/L group ( P ＞ 0.05 ) ;the different concentration of metformin at 24 h,48 h,72 h of each group were able to inhibit proliferation of VSMC,and there were statistically significant differences ( P ＜ 0.05 ) ; 10 -4 mol/L and 10 -3 mol/L of metformin group had the strongest inhibition compared with the others (P ＜0.05).Conclusion Metformin for VSMC proliferation of AGE induce had inhibited effect.
Keywords：MetforminAdanced glycation end productsCell proliferation
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)