The study on the protective effect of HSP70 induced by norepinephrine on the donor heart
GUO Qi-cang
ZHAO Chang-quan
WU Peng-yu
ZHANG Yu
CHEN Ling
CHEN Chen
PU Guo-hua
ZHANG Chun-qing
Abstract：Objective To investigate protective effect of endogenous Heat Shock Protein(HSP) 70 in vitro for donor heart,and to find a more effective method to extend the donor heart preservation by pretreatment of Norepinephrine.Methods Sixty rats were divided into two groups randomly( thirty in each group).Experimental group:SD rats were injected Norepinephrine by introperitoneal once every 24 hours,which were administered 6 times.Control group: SD rats were injected normal saline by introperitoneal once every 24 hours,which were administered 6 times.SD rats in each group were killed for collecting myocardium tissue after twenty-four hours from the last injection.The expression of myocardial HSP70,superoxide dismutase(SOD) activity and malondialdehyde(MDA) contents were checked by immunohistochemistry.Results Compared with the control group,the expression of HSP70 was higher in experimental group at the five time points [ ( 9.36 ± 2.53 ) ％ vs ( 3.59 ± 1.01 ) ％,( 9.09 ± 2.34) ％ vs (3.24±0.87)％,(8.65 ±2.11)％ vs(2.08 ±0.56)％,(6.99 ± 1.77)％ vs(1.06 ±0.26)％,(2.80 ±0.65 )％ vs(0.25 ±0.05 )％,P ＜0.01 ].In the experimental group,the SOD activity in the myocardial tissue decreased significantly after preservation of ten hours,which equaled to the six-hours in the control group.Compared with the control group,the contents of MDA in the experimental group had a lower expression at the five time points,which showed a continuous increasing tendency following the preserved time.In the experimental group,the MDA contents in the myocardial tissue after being preserved ten hours had a significantly increase at the five time points [ (0.349 ± 0.092 ) nmol/mg vs ( 1.98 ± 0.55 ) nmol/mg,( 0.349 ± 0.092 ) nmol/mg vs ( 2.13 ± 0.57 ) nmol/mg,(0.525 ± 0.102 ) nmol/mg vs ( 2.94 ± 0.55 ) nmol/mg,( 1.283 ± 0.241 ) nmol/mg vs ( 4.02 ± 0.97 ) nmol/mg,(2.70 ± 0.415 ) nmol/mg vs ( 5.31 ± 1.34) nmol/ mg,P ＜ 0.01 ].The activity of SOD in the experimental group had a higher expression at the five time points[ (250.87 ± 61.23) U/mg vs( 146.50 ± 40.37) U/mg,(246.36 ± 57.42) U/mg vs ( 143.28 ± 39.21 ) U/mg,(235.85 ± 54.33 ) U/mg vs ( 118.46 ± 35.81 ) U/mg,(205.61 ± 43.26 ) U/mgvs (79.37 ± 21.65 ) U/mg,( 112.03 ± 31.75 ) U/mg vs ( 50.26 ± 14.98 ) U/mg,P ＜ 0.01 ],which showed a continuous decreasing tendency following the preserved time.Conclusions The HSP70 expression in myocardium is high with Norepinephrine preconditioning,which is a beneficial protection for donor heart by decreased contents of MDA,increased SOD activity and increased myocardial anti-oxidation.
Keywords：HSP70NorepinephrineDonor heart myocardial protectionSODMDA
Publication Date：2012-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)