Evaluation on safety of different concentration of ozone on rats' knee joint tissue
ZHU Quan
WANG Xin
YAN Jin
XIAO Nian-lai
YANG Wei
SHU Yong-hui
Abstract：Objective To evaluate the pathological change of articular cartilage of knee joint in normal rat by different concentrations of ozone injection,oxidative stress degree of cartilage and synovial embrane were evaluated.Methods Thirty-two rats were randomly divided into A,B,C,D group.The O2-O3 mixed gas 1ml were injected into intraarticular with the concentration of 10 mg/L in group A,30 mg/L in group B,50 mg/L in group C,80 mg/L in group D.Biological examination was performed after 2 days,2 weeks and 4 weeks with the light microscope,se lecting the same locations of the other leg was used as control.Results Compared to the control,light microscope showed myocyte 10 mg/L in group A,cartilage and synovial membrane had not display obviously abnormality after 2 days,2 weeks and 4 weeks.Oxygenation cartilage and synovial 50 mg/L O2-O3 mixed was a little than 30 mg/L O2-O3 mixed gas.It is oxygenation 80 mg/L O2-O3 mixed gas more than it.Cartilage emerged different degree cytolysis.Moreover cartilage occurrence tissue and disorganization.Conclusions Since ozone concentration above 50 mg/L,which bring about damage to cartilage,synovial cell.80 mg/L concentration of ozone show up dlisorganization.However it is safe 30 mg/L concentration of ozone.
Keywords：OzoneArthrodial cartilageMembrana synovialisSafety
Publication Date：2011-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)