Effect of Eerdun-wurile on the serum nitric oxide and anti-oxidant enzymes activities of rabbits with athero sclerosis

DONG Ping; MA Chun-jie; HAN Xue-mei; WANG Bin

doi:10.3760/cma.j.issn.1673-4777.2011.11.006

Abstract：Objective To investigate the effects of Eerdun-wurile on the serum nitric oxide(NO) and antioxidant enzymes activities of rabbits with atherosclerosis.Methods An atherosclerotic rabbit model was established by feeding high fat diet.The 36 rabbits were randomly divided into 4 groups.Control group was fed with normal diet,the other 3 groups were fed high fat diet.The normal group and model group were administered distilled water.Positive control group received simvastatin 5 mg/( kg ? d),the Eerdun-wurile-treated group were given the Eerdunwurile 0.4 g/( kg ? d) continuous 90 days.The NO[(231.26 ±44.92) μmol/L]and the activities of superoxide dismutase (SOD)[(57.81 ± 7.65 ) U/ml ],catalase (CAT)[(25.71 ± 4.60) U/ml]and glutathione peroxides (GSH-Px) (515.55 ± 81.36)U/ml,as well as and the contents of malonaldehyde (MDA)[(22.27 ± 11.43)nmol/ml]in serum were measured.The contents of serum total cholesterol (TC),Triglyceride (TG),Low-density lipoprotein cholesterol (LDL-C),High density lipoprotein cholesterol(HDL-C) of rabbits in each of the groups were determined,and the area of rabbit aorta suffering from atherosclerosis plaque were measured.Results Compared with model group,Eerdun-wurile could increase serum NO content and SOD,CAT and GSH-Px activity(P ＜0.05 )and reduce the content of MDA in atherosclerosis rabbits( P ＜ 0.05 ) and the serum contents of TC,TG and LDL-C,as well as the area of lipid-plaque aorta endothelium of rabbits ( P ＜ 0.05 ).Conclusion Eerdun-wurile can protect vascular endothelial function by increasing levels of serum NO,improve the body's antioxidant capacity by enhancing the body's antioxidant enzyme activity,modulate the serum lipid level and prevent the development of atherosclerosis in the model.