Protective effects of alpha lipoic acid on diabetic skeletal myopathy by attenuation mitochondria-dependent cell apoptosis
ZHANG Qiu-mei
LI Chun-jun
ZHANG Jing-yun
LI Ming-zhen
YU De-min
Abstract：Objectives To explore the mechanisms of high glucose induced skeletal muscle cell apoptosis and observe the protection of antioxidant-lipoic acid(α-LA) on skeletal muscle. Methods Diabetic rat model was induced by a single dose of streptozotocin (45 mg/kg), randomly divided into 3 groups: normal control, diabetes control and α-LA. The structure and fibrosis of skeletal muscle were determined by HE and masson staining respectively. The protein level of cytochrome-c and Caspase-3 were detected by western blot. Results Twelve weeks after STZ-injection, there were significantly structural abnormalities and increased fibrosis in diabetic skeletal muscle,Caspase-3 protein level and mitochondrial cytochrome c release significantly increased in DM group versus control group. However, these diabetes-related skeletal dysfunctions were significantly ameliorated by antioxidant α-LA administration. Conclusions Our findings indicate that high glucose can increase mitochondrial cytochrome c release resulting in skeletal Apoptosis. The antioxidant-LA can effectively attenuate mitochondria-dependent skeletal apoptosis and exert a protective role against the development of diabetic skeletal myopathy.
Keywords：DiabetesLipoic acidMitochondriaSkeletal myopathy
Publication Date：2011-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)