Quality control of serum matrix metalloproteinase-9 detection in patients with coronary artery disease
DONG Lei
LIU Sa
WANG Lu-ya
Abstract：Objective To evaluate the "Instant Method" in enzyme-linked immunosorbent assay (ELISA)by detecting matrix metalloproteinase-9 (MMP-9) and to discuss the quality control in the serum in patients with coronary artery disease (CAD). Methods The level of MMP-9 in the serum of patients with CAD was measured by MMP-9 ELISA Kit, and "Instant Method" was used for internal quality control. Results MMP-9 levels were measured in MMP-9 ELISA Kit and the coefficient of variance (CV) was no higher than 15%. The value of SI between 0.625 and 1.15 was in the acceptable range. The level of MMP-9 significantly increased in the serum of the patients with myocardial infarction compared with the health control and angina pectoris. Conclusion "Instant Method" is an effective approach for internal quality control.
Keywords：Matrix metalloproteinasesEnzyme-linked immunosorbent assay"Instant method"quality control
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)