Erythrocyte native immune adhering function in patients with myelodysplastic syndrome and its effect on natural killer cell killing activity
SHANG Bao-jun
Abstract：Objective To investigate the change of native immune adhering function (ENIAF) inself-plasma of patients with myelodysplastic syndrome and its effect on the killing activity of natural killer ( NK ) cells.Methods The whole blood was anticoagulated with citric acid. 5 μl precipitated red blood cells and 500 μl plasma of patients or controls were directly mixed with 750 μl quantitative K562 cells at 37℃ for 30 minutes. One K562 cell attached by one or more erythrocytes was counted as one rosette,the ratio of rosettes was calculated. Using K562 cells as target cells, the killing activity of NK cells isolated from normol persons was detected by MTT assay, the change of the killing activity was observed after adding RBCs. Results The ratio of rosettes formed by RBCs of 21 normal controls and K562 cells was (15.6 +6.7 )%, and the ratio of rosetfes formed by RBCs of 16 patients and K562 cells was (8.5 ±7.0)%. The ability of ENIAF in patients with myelodysplastic syndrome was significantly lower than that in healthy individuals( t = 3.66 ,P ＜ 0. 01 ). The killing rate of NK cells in peripheral blood of normal individuols was 64%-75% without adding RBCs, and it increased to 79%-88% after adding RBCs. The test group was 45%-56% without adding RBCs, and it increased to 51% -58% after adding RBCs. Conclusions It is concluded that the ENIAF of RBCs in patients with myelodysplastic syndrome decreases, accompanying the reduction of the killing activity of NK cells to K562 cells. To detect change of ENIAF may be helpful for the assessment of the immunological function of patients with myelodysplastic syndrome.
Keywords：Myelodysplastic syndromesErythrocyteInnate immune adhering functionNatural killer cell
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)