Effect of lentivirus-mediated shRNA targeting collagen type Ⅰ gene on biological behaviors of rat mesangjal cells
SHAN Yi
ZHOU Chun-hua
ZHAO Hai-dan
Abstract：Objective By observing the change of proliferation, apoptosis and cell cycle about RMC infec-ted by lentivirus-mediated COL I shRNA, we explore the physiological impact on the target cells by RNAi medicine .Methods We observed the infected RMC by Infection enhancer( control group),lentivirus vector( pSC-GFP group) and lentivirus-mediated COL Ⅰ shRNA (pSC-GFP/COL Ⅰ group) ,cell proliferation was measured with MTT method,apoptosis was measured with Annexin V/PE method,cell cycle was measured with flow cytometry and the impact on the above-mentioned ability of cells infected by lentiviral expression vector was observed. Results Both pSC-GFP/COL Ⅰ group and pSC-GFP group can significant inhibit cell proliferation. Suppression ability of pSC-GFP group was significantly higher than pSC-GFP group. 24 hours,48 hours and 72 hours after infection showed inhibition efficiency of 22.52% , 28.57% , 33.33% , respectively. The result of apoptosis show that apoptosis was induced to a certain degree after lentivirus-mediated shRNA infected RMC, but RNAi didnt induce further aggravation. The result of cell cycle experiment showed that lentivirus induces G2/M cell cycle arres. To 72 houres after RMC infected by lentiviral expression vector, increasingly prominent showed that COL Ⅰ shRNA induced S cell cycle arrest. Conclusions Af-ter transfection of rat mesangial cells, COL Ⅰ shRNA affects cell proliferation at a certain degree. But on the whole,its a safe way to lay the foundation for further animal experiment and drug development.
Keywords：Cell cycleLentivirus vectorCell proliferationApoptosisCollagen type Ⅰ
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)