High performance liquid chromatogaph determination of lonidamine's related substance and content
JIN Chong
XU Ning
CHEN Jing
ZHOU Guo-fang
JIAO Xiao-li
YU Xiao-yan
Abstract：Objective To observe the impurities and assay of Lonidamine.Methods Based on the system suitability and specificity, a reversed-phase column [Hypersil C18 column silane (ODS) (200 × 4.6 mm)] and a methanol-water-glacial acetic acid (80: 11:1) mobile phase, Lonidamine's related substances and content were determined by HPLC with the column temperature 25℃, the flow rate 1.0 ml/min and the detection wave length 298 nm.Results Lonidamine is linear relationship with its peak area in in the concentration range of 2 ～ 16 μg/ml,its regression equation is A = 7043C-108353, r = 0.9914. All the contents were above 98.5%. Conclusion Determination of lonidamine related material and content by high performance liquid is accurate, simple, feasible,as well as of high reproducibility and stability.
Keywords：High performance liquid chromatographyLonidamineRelated substancesDetermination assay
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)