Construction of eukaryotic expression vector of miRNA-1 and the expression of green fluorescent protein in transfected bone marrow mesenchymal stem cells
WEN Tong
WEI Yun-feng
WANG Meng-hong
ZHENG Ze-qi
PENG Jing-tian
ZENG Yuan-lin
ZENG Jun-yi
Abstract：Objective To construct an eukaryotic expression vector for mo-miRNA-1 to provide the experimental basis for miRNA-1 functions.Methods miRNA-1 genomic sequence was amplified by polymerase chain reaction(PCR) and cloned into pSD11-U6/Neo/GFP plasmid.Positive clones were identified with PCR and DNA sequencing, then mesenchymal-like stem cell were transfected pSD11-U6/Neo/GFP/miRNA-1 recombinant plasmid with Lipofectamine.Expression of green fluorescent protein was observed under a fluorescence microscope.Results PCR identification and DNA sequencing showed that the eukaryotic expression vector for miRNA-1 constructed successfully, green fluorescent protein expression was detected after transfection.Conclusion The eukaryotic expression vector for mo-miRNA-1 can be constructed successfully.
Keywords：Bone marrow mesenchymal stem cellsmiRNA-1Eukaryotic expression vector
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)