Screening serum protein markers from patients with lymphoma
JIANG Wei
WANG Kai-zheng
TANG Xue-qing
CAI Mei-zhu
ZHOU Ming-shu
Abstract：Objective To evaluate the diagnostic significance of serum protein markers in patient with lymphoma.Methods The sera of 81 patients with lymphoma and 86 healthy controls were analyzed by SELDI-TOF-MS and CM10 protein chips.The profiling was used for identifying the statistically significant peaks as well as for developing predictive model based on biomarker patterns software (BIAS) and artificial neural network (ANN).Results A total of 65 protein peaks were obtained and 39 differential peaks were selected (P＜0.05).A diagnostic model constructed by BPS with proteins of m/z 4 359,6 673,8 978 and 15 190 could successfully diagnose lymphoma with sensitivity of 92.7% and specificity of 89.1%.The ANN model based on the four markers distinguishing lymphoma between healthy controls had a sensitivity of 87.8% and a specificity of 87.0%.Conclusions Using standard protein as a control material can improve the experimental reproducibility.This strategy is a potential method for rapid diagnosis of lymphoma.
Keywords：LymphomaSELDI-TOF-MSProtein marker
Publication Date：2010-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)