Influence of mineral water rich in microelement on proliferation of vascular smooth muscle cells

ZHAO Li-min; WEN Jie; LIU Kuo; LIU Jie-lin; LIU Ya; HONG Zhao-guang; TANG Chao-shu; WEN Shao-jun

doi:10.3760/cma.j.issn.1673-4777.2010.03.001

Abstract：Objective To study the influence of mineral water rich in microelement on proliferation of human vascular smooth muscle cells(HVSMCs) in vitro. Method Ten samples of human great saphenous vein of patients undergoing coronary artery bypass surgery were obtained. Two kinds of DMEM media including Jinchuan mineral water (MW-DMEM)and double distilled water(DDW-DMEM) were observed. The free tissue formation time and the "peak-valley" time of HVSMCs cultured in these 2 kinds of DMEM media were observed and recorded.A cell proliferating model of HVSMCs was induced by angiotensin Ⅱ. 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny-ltetrazolium bromide (MTT) was used to determine the cells growth. The cell was confirmed to be HVSMCs by im-munohistochemistry and electromicroscopy analysis. Results The HVSMCs in MW-DMEM group emigrating from the tissue explanted within (3.7±0.2) days while the HVSMCs in DDW-DMEM group explanted within (8.5±0.6)days. The time of HVSMCs forming a 'peak-trough state' from a adherence state in MW-DMEM group was (8.3±0.2)days and it was (7.1±0.5)days in DDW-DMEM group. The result showed that H VSMCs proliferation induced by Ang Ⅱ could be inhibited by Jinchuan mineral water. HVSMCs were confirmed under electron microscope of the integrity of cell morphology and the surface is rich in microvilli. HVSMCs cytoplasm was rich in myofilaments and was in a longitudinal and parallel arrangement in intracellular dense areas Anti-α-actin antibodies with immuno-histochemical staining showed lots of stained buffy bundles in cell cytoplasm. Conclusions The results show that Jinchuan mineral water rich in microelement and mineral can relieve injury of HVSMCs. Meanwhile HVSMCs prolif-eration induced by Ang Ⅱ can be inhibited by Jinchuan mineral water.