Specific cycotoxic effects against hepatocellar carcinimar by fusing hnman dendritic cells with heptic cancer cells
YIN Fang
ZOU Jin-lin
LU Bao-jun
MO Xiang-qiong
WENG Ze-bin
TANG Cong
Abstract：Objective To investigate the effect of fusions of hepatic cancer cell(HCC) with human dendrit-ic cell (DC) induce autologous T lymphocytes on eliciting specific immunity against HCC. Methods DC isolated from human peripheral blood were cultured and proliferated for one week by using recombinant human granulocyte/macrophage-colony stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4). Fusions of DC with HepG2 cells (HepG2/DC) were achieved by pelythylenegiycol (PEG). The ability of HepG2/DC to stimulate proliferation and differentiation of autologous T lymphocytes was assessed by MTT method and the specific killing efficacy of HepG2/DC-induced cytotoxic T lymphocytes (CTL) to HepG2 was evaluated. Results After one week culture, DC present a high level expression of CD1a, HLA-DR, CDS4, CD80, CD86. Fusions had remarkably greater ability to stimu-late proliferation of autologous T lymphocytes in compare with HepG2, HepG2 + DC and DC. The HepG2/DC acti-vated CTL showed a potential specific killing efficacy to HepG2. Conclusion Fusions of HCC cells with DC can ef-fectively stimulate autologous T lymphocytes to elicit specific antitumor immunity against HCC.
Keywords：Dendritic cellHeptic cancer cellFusion
Publication Date：2009-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 842-844 )