Detection of Epstein-Barr virus DNA in peripheral blood mononuclear cells in children of infectious mononucleosis
FU Li-juan
ZHAO Hong-wei
XU Yan-mei
Abstract：Objective Fluorescent Quantitative PCR was used to determine Epstein-Barr virus (EBV) DNA in peripheral blood mononuclear cells (PBMC) of patients with infectious mononucleosis (IM). Methods EBV DNA in 38 patients with IM were detected by Fluorescent Quantitative PCR and EBV VCR-IgM in plasma of peripheral blood were detected by Enzyme-Linked Immunoserbent Assay (ELISA) at seven days, one month, three months, six months and nine months after setup. Results At 7 days after setup, the positive rate of EBV DNA in PBMC was 89.0 % (34/38) and that of EBV VCR-IgM was 57.9 % (22/38). The positive percent in PBMC was significant higher than that in EBV VCR-IgM (P < 0.05 ). The positive rate of EBV-DNA in peripheral blood was 42.1% (16/38), showing no difference from EBV VCR-IgM in plasma(P > 0.05 ). The positive rate of EBV-DNA in PBMC was significant different from that in plasma (P < 0.05 ). 2 cases of EBV DNA positive in plasma and 17eases of EBV DNA positive in PBMC were found at 1 month after setup. The results of EBV DNA in plasma were negative. Conclusion EBV DNA in PBMC may be an early, rapid and sensitive diagnosis method on IM in the early stage of EBV infection. EBV DNA in PBMC detected by Fluorescent Quantitative PCR is an effective technique of determining therapy and monitoring disease progress. EBV infection in PBMC may be related to systematic injury in patients of infectious mononucleosis.
Keywords：Infectious mononucleosisEpstein-Barr virus DNAPeripheral blood mononuclear cellsFluorescent quantitative polymerase chain reactionEnzyme-linked immunoserbent assay
Publication Date：2009-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 678-680 )