Effects of sodium ferulae on vascular morphologic indexes of pulmonary, cell proliferation and apoptosis in chronic hypoxic rats

HAN Ji-chang; ZHANG Yi-jie; WEI Yu-ju; LI Si-hong; WANG Bo-zhang

doi:10.3760/cma.j.issn.1673-4777.2009.09.012

Abstract：Objective To investigate the effect of sodium ferulate on vascular morphologic indexes of pul-monary,expression of p53 and PCNA, cell proliferation and apoptosis in chronic hypoxic rats. Methods Thirty healthy Spraque-Dawley male rats were randomly divided into three groups: normal control group (n = 10), chronic hypoxic group (n = 10) and hypoxia plus sodium ferulate treatment group (n = 10). The model of chronic hypoxic pulmonary hypertension was established by exposing rats to normobaric hypoxic conditions for 8 hours one day in 3 weeks continually. Effects of sodium ferulate on vascular morphologic indexes of pulmonary, expression of P53 and PCNA, cell proliferation and apoptosis in pulmonary tissue of HPH rats were observed. Results After 21 days, rats in chronic hypoxic group in MT%, MA%, PI, expression of p53 are obviously higher than normal control group (P < 0.01 ), in VA%, AI are distinctly higher than normal control group (P < 0.01);Rats of hypoxia plus sodium ferulate treatment group in MT%, MA%, PI and expression of p53 are obviously decreased than chronic hypoxic group (P < 0.01 or P < 0.05 ), in VA% and AI are increased higher than chronic hypoxic group (P < 0.01);Ex-cept that VA% ,AI and expression of p53 have meaning (P < 0.01 or P < 0.05 ), other indexes have no meaning compared with normal control group (P > 0.05). Conclusion Sodium ferulate can refrain persistently from the de-velopment of pulmonary vascular remodeling efficiently in chronic hypoxic rats. The mechanism may be related to in-hibiting the protein expression of p53 and PCNA, enhancing cell proliferation and decreasing cell apoptosis.