The induced expression of AmpC gene in pseudomonas aeruginosa biofilms
ZHAO Jing-ming
CHENG Wei
JIANG Han-dong
Abstract：Objective To study the expression of AmpC gene in Pseudomonas aeruginosa induced by antibiotics at plank tonic and biofilms phases. Methods An in vitro model of PAO1 biofilms was established with modified flat-board method. Being induced by antibiotics at plank tonic and biofilms phases, the expression of AmpC gene in PAO1 was quantified by real-time quantitative PCR. Results Without the effect of antibiotics, the expres-sion of AmpC gene in PAO1 was low at both plank tonic and biofiims phases. Being induced by imipenem and eeftazidime that can induce the maximal AmpC β-lactamase activities, the expression of AmpC gene in biofilms PAO1 was higher than that in plank tonic PAO1. During both plank tonic and biofilms phases, the expression of AmpC gene in PAO1 induced by imipenem was higher than that induced by ceftazidime. Conclusion Induced by antibiotics,biofilms PAO1 showes stronger ability for the expression of AmpC gene than plank tonic PAO1. The ex-pression of AmpC gene induced by imipenem is higher than that induced by ceftazidime.
Keywords：Pseudomonas aeruginosaBiofilmsAmpCGeneReal-time quantitative polymerase chain reaction
Publication Date：2009-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 641-643 )