Construction of recombinant adenoviral vector carrying NOD2
LU Hui-xia
LI Shao-bo
Abstract：Objective To construct adenovirus vector containing the NOD2 gene for studying therapeutic inflammation disease. Methods NOD2 was obtained from human CoCa2 DNA based on Adopt molecule clone tech-nique, and then was cloned into plasmid pcDNA3.1 (+) and further cloned into plasmid pShutde-CMV ,pShuttleC-MV-NOD2 with double digestion of PI- Kpn Ⅰ and Ⅰ- Not Ⅰ. The recombinant adenoviral plasmid was identified and transferred into the adenoviral packaging cell HEK293 by lipofectamine 2000 mediated gent transfer method. The recombinant adenovius was confirmed by polymerase chain reaction (PCR). Results The recombinant pAdeno-NOD2 was correctly constructed and confirmed by restriction endonuclease analysis. The transferred HEK 293 cells were lysed by freezethawingto obtaining the recombinant adenovirus in the lysate. The PCR product of the lysate confirmed the presence ofrecombinant adenovirus. Conclusion The recombinant adenovims containing the NOD2 gene can be successfully constructed, which provides the further foundation of NOD2 gene therapy for inflammation disease.
Keywords：Adenovirus vectorNucleotide-binding oligomerization domainNucleotide-binding oligomerization-2
Publication Date：2009-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 501-503 )