Plasma and tissue p16 gene mutation in non-small cell lung cancer
TAN Wei
GUO Gui-fang
YU Zhen-gang
Abstract：Objective To study the significance of plasma and tissue p16 gene mutation in non-small cell lung cancer (NSCLC) patients. Methods Polymerase chain reaction (PCR) amplification exons 2 of p16 gene and single-stranded conformation polymorphism (SSCP) mutation. Results In 32 NSCLC patients, p16 gene muta-tions were found 14 in plasma (43.75%) and 17 in tissue(53.10% ). NSCLC patients with plasma and tissue p16 gene mutation in Ⅲ + Ⅳperiod was significantly higher than that in Ⅰ + Ⅱ period (P>0.05). The rate of Lymph node metastases was significantly higher than that without lymph node metastasis (P>0.05 ). Conclusion Plasma DNA p16 gene mutation in the second exon in NSCLC shows a higher detection rate. The detection rate of plasma DNA p16 gene in the second exon mutation is similar to that of the tumor tissues. The plasma DNA p16 gene in the second exon mutation of NSCLC patients is related with tumor clinical stage and lymph node metastasis. Detection of the plasma p16 gene mutation of NSCLC patients has important value for diagnose and prognosis estimation of NSCLC.
Keywords：Non-small cell lung cancerp16 genePlasmaTissueMutation
Publication Date：2009-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 262-264 )