Reversal effect of Tetredrine(TTD)on human hepatocellular carcinoma(HCC)multidrug-resistant cell line (Hep-3B/ADM)
SUN Yu
HE Ke-jian
GONG Mei-jin
Abstract：Objective To investigate reversal effect on Hep-3B/ADM by TTD and its mechanism.Methods An adriamycin-resistant human HCC cell line(Hep-3 B/ADM)was established.through exposure to gradually increased concentration of ADM.Drug sensitivity was measured by MTT.Flow cytometry(FCM)was performed to assess the expression of P-170 and the fluorescene intensity of Rh13 in the cells.Results TTD(0.10-1.00 μmoL/L)can reverse multidrug-resistant of Hep-3B/ADM.TTD decreased the expression of P-170 in the cells significantly.Conclusion TTD significantly enhancs ADM-induced cytotoxicity.The mechanism is mediated by inhibiting muhidrug-resistant(MDR1).
Keywords：Hepatocellular carcinomaTetredrineAdriamycinMuhidrug-resistant
Publication Date：2008-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：2( 790-791 )