Affects of high energy shock wave on Ca2+-ATPase activity in rabbit renal tissues with and protective effects of ligustrazine
GUI Xi-qing
GUO Zhen-yu
SUN Hua-bin
ZHENG Wei
LIAN Wen-fei
WEI Bin
Abstract：Objective To observe the effects of high energy shock wave on Ca2+-ATPase activity in rabbit renal tissues with and protective effects of ligustrazine. Methods Thirty healthy rabbits were made into the mono-nephron models and then divided into 3 groups: control group, HESW group and HESW plus ligustrazine pretreated group. Rabbit kidney tissues were analyzed 24 hours after HESW. The activity of Ca2+-ATPase in cell membranes and mitochondriat fractions in renal tissue were analyzed with optical method. Cell apoptosis was detected by TdT-mediated dUTP Nick End Labelling (TUNEL) and flow cytometry. The variations of creatinine clearance (CCr) were observed.Results Compared with the control group, The activity of Ca2+-ATPase in cell membranes and mitochondrial fractions reduced, the rate of apoptosis increased and CCr reduced significantly in the HESW group. The activity of Ca2+-ATPase in cell membranes and CCr showed no significant difference between the HESW and ligustrazine pretreated group and the control group. The activity of Ca2+-ATPase reduced in mitochondrial fractions (P＜0.05)and cell apoptosis rate were significantly higher (P＜0.05) in the HESW plus ligustrazine pretreated group than the control group, but showed the highest level in the HESW group. Conclusion Ca2+-ATPase reduction and cell apoptosis increases are important mechanisms in the kidney function damage caused by HEWS. Lignstrazine can improve kidney function by suppressing kidney Ca2+-ATPase activity reduce and cell apoptosis.
Keywords：Kidney damageHigh energy shock waveCa2+-ATPaseLigustrazine
Publication Date：2008-01-01
Online Publishing Date：2026-09-14(First online date of this platform, not the publication date of the document)
Pages：3( 400-402 )