Establishment of enzalutamide-resistant human prostate cancer cell lines and screening of lncRNA and mRNA expression profiles
GUAN Han
LING Zhi-xin
FANG Fang
MAO Li-kai
YOU Zong-hao
WANG Can
CHEN Shu-qiu
XU Bin
CHEN Ming
Abstract：Objective:To establish enzalutamide-resistant human prostate cancer cell lines and screen out the lncRNA and mRNA expression profiles associated with enzalutamide resistance.Methods:Human prostate cancer cell lines LNCAP and C4-2B were cultured with 10 μmol/L enzalutamide for 6 months in vitro for the establishment of enzalutamide-resistant subclones LNCAP-ENZA and C4-2B-ENZA.The IC50 value and enzalutamide resistance index of each cell line were examined by MTT assay,the expressions of enzalutamide-related genes FL-AR,AR-V7 and HnRNPA1 were determined by Western blot,and the lncRNA and mRNA differential expressions of C4-2B and C4-2B-ENZA were detected by high-throughout lncRNA microarray.Results:Compared with LNCAP and C4-2B,the IC50 values of enzalutamide-resistant subclones LNCAP-ENZA (60.83 μmol/L) and C4-2B-ENZA (88.32 μmol/L) were increased significantly (P ＜ 0.05) and the enzalutamide-resistance indexes of the LNCAP-ENZA and C4-2B-ENZA cells were 4.94 and 4.67,respectively.The expressions of AR-V7 and HnRNPA1 were markedly up-regulated in the LNCAP-ENZA and C4-2B-ENZA cells as compared with those in the LNCAP and C4-2B cells,but that of FL-AR showed no significant change.A total of 1 440 lncRNAs and 1 236 mRNAs were identified as differentially expressed in the C4-2B-ENZA cells.Conclusion:Enzalutamide-resistant human prostate cancer cell subclones LNCAP-ENZA and C4-2B-ENZA were successfully established and enzalutamide resistance-associated lncRNA and mRNA were identified,which may provide some molecular evidence for the management of enzalutamide-resistant human prostate cancer.
Keywords：prostate cancerenzalutamide resistanceLNCaP cell lineC4-2B cell linelncRNAmRNA
Publication Date：2018-01-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 116-121 )