Establishment of a rat model of premature ejaculation with 8-OH-DPAT

GUO Jun; YAN Bin; WANG Fu; GAO Qing-he; ZHANG Xiu-ju; YU Guo-jin; ZENG Yin; QIU Jun-feng; GENG Qiang; HAN Qiang

doi:10.13263/j.cnki.nja.2018.02.002

Abstract：Objective:To explore the feasibility and practicability of establishing a rat model of premature ejaculation (PE) by injection of 8-OH-DPAT into the subarachnoid space of the lumbosacral spinal cord segments.Methods:Twenty-four male Wistar rats were equally randomized into a PE model and a blank control group.The PE model was established by injection of 8-OH-DPAT in 10 ml normal saline at 0.8 mg per kg of the body weight per day into the subarachnoid space of the lumbosacral spinal cord segments and the control rats were injected with the same volume of normal saline only,both for 4 weeks.Another 24 female Wistar rats were injected subcutaneously with benzoic acid estradiol at 20 μg to induce estrus at 36 hours before mated with the male animals.At 2 and 4 weeks,the male rats were mated with the female ones for 30 minutes each time and meanwhile observed for their mating behavior indicators,such as mount latency,intromission latency,ejaculation latency,mount frequency,intromission frequency,and ejaculation frequency.Results:Compared with the controls,the PE model rats showed a significantly lower ejaculation latency ([712.35 ± 36.77] vs [502.35 ± 46.72] s,P ＜ 0.05),mount latency ([11.22 ± 3.60] vs [8.69 ± 2.48] s,P ＜ 0.05),mount frequency (13.28 ± 0.24vs7.53 ± 1.84,P＜0.05),andintromissionlatency ([22.33 ± 2.45] vs [12.08 ± 1.39] s,P＜0.05),but a remarkably higher ejaculation frequency (2.01 ± 0.48 vs 4.26 ± 0.89,P ＜ 0.05).No statistically significant difference was observed between the control and model animals in the intromission frequency (7.49 ± 2.21 vs 6.45 ± 1.89,P ＞ 0.05).Conclusion:A rat model of premature ejaculation was successfully established by injection of 8-OH-DPAT into the subarachnoid space of the lumbosacral spinal cord segments,which is of great significance for further study of the mechanism of premature ejaculation.