Sperm DNA damage is not related to seminal leukocyte subsets in infertile males with asymptomatic genital tract infection
LIU Xu-chen
CHEN Guang-she
HE Xiao-long
Abstract：Objective:To study the correlation of leukocyte subsets with sperm DNA damage in the semen of infertile men with asymptomatic genital tract infection (AGTI).Methods:This study included 111 infertile males with AGTI.After routine semen analysis,we determined the concentration of CD45 + leukocytes in the semen by immunocytochemistry,measured the concentrations of CD14 + cells of the mononuclear/macrophagic system and activated macrophages and HLA-DR + cells in the semen by flow cytometry,and examined the sperm DNA fragmentation index (DFI) and the expression of 8-hydroxy-2'-deoxyguanosine (8-OHdG) by TUNEL assay.Then we analyzed the correlation of seminal leukocyte subsets with sperm DNA damage and routine semen parameters.Results:The concentration of CD45 + leukocytes was correlated significantly with those of CD14 + and HLA-DR + cells in the semen (P ＜ 0.01),but not that of leukocyte subsets with routine semen parameters,sperm DFI,or the percentage of 8-OHdG-positive cells.The percentage of 8-OHdG-positive sperm was correlated positively with the sperm DFI (r =0.48,P ＜ 0.01) but negatively with sperm concentration (r =-0.44,P ＜0.01).After adjusted for age,abstinence time and cigarette smoking,the percentage of 8-OHdG-positive sperm was correlated independently with sperm concentration (β =-0.25,P =0.008) and DFI (β =0.23,P =0.05).Conclusion:Sperm DNA damage is associated with poor semen quality but not with seminal leukocyte subsets in infertile males with asymptomatic genital tract infection.
Keywords：asymptomatic genital tract infectionleukocyte subsetCD45CD148-hydroxy-2'-deoxyguanosinesperm DNA damage
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 45-49 )