Application value of frozen-thawed mouse round spermatids in in vitro fertilization
JIANG Hong
WANG Li
WANG Cun-li
ZHANG Wen-xiang
NI Feng
Abstract：Objective:To investigate the application value of the frozen-thawed round spermatids of the mouse in in vitro fertilization (IVF).Methods:Haploid spermatids of the mouse obtained in vitro were divided into a frozen-thawed and a fresh group and oocytes were collected from 6-8 weeks old female mice.After diamidino-phenyl-indole (DAPI) staining,the oocytes were subjected to intracytoplasmic round spermatid injection (ROSI),259 in the frozen-thawed and 238 in the fresh group.Comparisons were made between the two groups in the capacities of fertilization and embryonic development.Results:The survival rate of the frozen-thawed haploid spermatids was (75.9 ± 2.3) ％.No statistically significant differences were observed between the frozen-thawed and fresh groups after ROSI in the rates of fertilization (51.9 vs 55.7％,P ＞ 0.05),2-cell embryos (51.0 vs 62.2％,P ＞ 0.05),4-8-cell embryos (41.8 vs42.9％,P ＞0.05),or morula-blastocysts (12.2 vs 21.4％,P ＞0.05).Conclusion:Frozen-thawed round spermatids of the mouse are similar to fresh ones in their capacities of fertilization and embryonic development.
Keywords：round spermatidin vitro fertilizationfreezing-thawingmouse
Publication Date：2017-12-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1059-1062 )