Effect of silencing the VDR gene on the migration and invasion of prostate cancer cells
ZHANG Yuan-dong
ZHAO Hui
LI Kang-jian
GUAN Run-yun
Abstract：Objective:To investigate the effect of small interfering RNA silencing the vitamin D receptor (VDR) on the biological behavior of prostate cancer PC-3 cells.Methods:We constructed the VDR-shRNA lentiviral vector and determined the mRNA and protein expressions of VDR by RT-PCR and Western blot.Using scratch wound healing and Transwell chamber assays,we detected the changes in the migration and invasiveness of the PC-3 cells after silencing VDR.Results:The VDR-shRNA plasmid significantly interfered the VDR expression and successfully screened the cell lines with stable VDR-shRNA interference.The rate of scratch wound healing was markedly lower in the VDR interference group than in the blank control and LV3 negative control groups (59％ vs 73.6％ and 77.8％,P ＜ 0.05),but with no statistically significant difference between the latter two (P ＞ 0.05),and so was the count of permeable cells (P ＜ 0.05),but with no significant difference between the latter two groups,either (P ＞ 0.05).The migration ability and invasiveness of the VDR-treated cells were remarkably decreased as compared with those of the control cells.Conclusion:Down-regulated expression of the VDR gene may reduce the migration and invasiveness of prostate cancer cells.
Keywords：PC-3 cellshRNAvitamin D receptorinvasivenessgene interference
Publication Date：2017-11-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 969-974 )