Expression characteristics of the USP24 gene in the mouse testis during spermatogenesis
HU Qi-yi
DENG Qiong
ZHANG Jian-wen
ZHI Fan
SUN Rui
LIANG Hui
Abstract：Objective:To investigate the expression characteristics of the USP24 gene in the mouse testis and its role in spermatogenesis.Methods:We examined the expression characteristics of USP24 in the testis tissues of wild-type mice at different postnatal weeks (PNW) and androgen receptor (AR)-knockout (ARKO) adult mice using real-time quantitative PCR and immunofluorescence,and detected the transcriptional activity of the USP24 promoter by dual-luciferase reporter gene assay.Results:The expression of the USP24 gene was low in the testis tissue of the wild-type mice at PNW 1,increased dramatically at PNW 3 and stayed at a similar level till PNW 8.The USP24 protein was located mainly in the cytoplasm of Sertoli and spermatogenic cells.Compared with the wild-type,the adult ARKO mice showed a decreased expression of USP24 localized in the posterior head and mid-piece of the mature sperm in the testis.Dual-luciferase reporter gene assay showed that the transcriptional activity of the USP24 promoter was increased after testosterone stimulation.Conclusion:The increased expression of the USP24 gene was associated with the initiation of sexual development,and the USP24 protein was expressed in the mature sperm of the mice.USP24 is an AR-target gene,which may be involved in the regulation of spermatogenesis in mice.
Keywords：ubiquitin-specific protease 24spermatogenesistestismouse
Publication Date：2017-11-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 963-968 )