Chinese medicinal compound CFF-1 induces the apoptosis and cycle-arrest of prostate cancer cells via the PI3K/AKT/FOXO1 signaling pathway
ZHANG Yang
WU Zhao-meng
LEI Bo-han
LU Zi-jie
ZHU Qing-yi
XU Fu-song
ZHANG Mao-sen
LIU Ping
Abstract：Objective:To explore the apoptosis-inducing effect of the Chinese medicinal compound CFF-1 on prostate cancer cells and its related molecular mechanisms.Methods:Normal prostate WPMY-1 cells and prostate cancer LNCaP,CWR22Rvl,PC3 and DU145 cells were treated in dehydrated alcohol with CFF-1 at 0,2,5,or 10 mg/ml for 24 hours.Then the viability of the prostate cells was detected by morphological observation,MTT and CCK-8 assay,nuclear condensation and disruption measured by DAPI staining,the cell cycle and apoptosis calculated by flow cytometry,the activity of the PI3K/AKT/FOXO1 signaling pathway and the expressions of its downstream apoptosis-and cycle-related proteins determined by Western blot.Results:CFF-1 significantly arrested the cell cycle in the G1 phase,decreased the cell viability and increased the nuclear condensation and disruption in a dose-dependent manner,and elevated the apoptosis rate of prostate cancer cells.At the molecular level,CFF-1 dose-dependently reduced the activity of the PI3K/AKT signaling pathway and phosphorylation of the FOXO1 protein,increased the transcription activity of FOXO1,and eventually regulated the expressions of cell apoptosis-and cycle-related genes.Conclusion:The Chinese medicinal compound CFF-1 can significantly inhibit the growth,arrest the cycle,and induce the apoptosis of prostate cancer cells by decreasing the activity of the PI3K/AKT/FOXO1 signaling pathway,which suggests its potential clinical application value in the treatment of prostate cancer.
Keywords：CFF-1apoptosiscell cyclePI3K/AKT/FOXO1 signaling pathwayprostate cancer
Publication Date：2017-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：10( 828-837 )