Excessive fluoride increases the expression of osteocalcin in the mouse testis
QIN Jian-hua
DILINUER Aierken
SAIMIRE Shataer
KALIBINUER Aierken
YUSUFU Mahemuti
YIRIZHATI Aili
CUI Sai-sai
NUERSIMANGULI Maimaitiming
CHEN Wu-jin
BAI Sheng-bin
Abstract：Objective:To observe the influence of excessive fluoride on the levels of osteocalcin and testosterone in the testis of the male mouse.Methods:Twenty-four C57BL/6J male mice were equally randomized into a normal control and a fluorosis model group,the former fed on distillcd water while the latter on a solution of sodium fluoride (100 mg/L) in distilled water,both for 12 weeks.Then,the level of osteocalcin in the testis tissue was measured with the immunohistochemical streptavidin-peroxidase (SP) method and those of osteocalcin and testosterone in the serum determined by ELISA.Results:After 12 weeks of fluoride intervention,the level of serum osteocalcin was significantly higher in the fluorosis models than in the normal controls ([68.05 ± 5.32] vs [47.50 ± 5.73] pg/mL,F =11.901,P =0.008),while that of testosterone markedly lower in the former than the latter group ([8.07 ± 1.35] vs [12.94 ± 3.09] ng/mL,F =2.313,P =0.006).The results of immunohistochemical SP showed the expression of osteocalcin in the cell membrane and cytoplasm of the fluorosis models,which was evidently higher than in the normal controls.Conclusion:Twelve-week intake of 100 mg/L fluoride solution can decrease the level of testosterone and increase the expression of osteocalcin in the testis of the male mouse.
Keywords：fluoridetestisosteocalcintestosteronemouse
Publication Date：2017-09-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 782-785 )